Trulieve at 1527 Northwest 6th Street in Gainesville, Florida, operates as a Florida-licensed Medical Marijuana Treatment Center (MMTC) in the 32601 zip code in downtown Gainesville — serving Alachua County’s most densely populated urban core and the patient communities of Gainesville’s northern neighborhoods including Pine Park, Oakview, Florida Park, Cedar Grove, Northwood, and Spring Tree. Gainesville is home to the University of Florida, one of the largest public universities in the United States with approximately 60,000 students, and to UF Health (Shands Hospital), one of Florida’s largest and most complex academic medical centers — two institutions that together create a patient population defined by academic, medical, and healthcare-adjacent demographics distinct from the Panhandle and Jacksonville markets to the north. The NW 6th Street location opens at 8:30 AM Monday through Saturday, among the earliest in the Gainesville MMTC market, and operates seven days a week. Trulieve carries its full in-house brand portfolio and Florida-exclusive partner brands across flower, vapes, edibles, tinctures, topicals, concentrates, and RSO, with online ordering for pickup and home delivery throughout the Alachua County service area. Delivery carries a $50 minimum with a $15 fee on orders below $100, and free delivery for qualifying patients 65 and older. Payment accepted via cash, Canpay, and an on-site ATM.

Does Trulieve Gainesville require a Florida medical marijuana card? Yes — Trulieve operates as a Florida MMTC under OMMU licensing, and a valid Florida Medical Marijuana Card is required for all purchases.

Does Trulieve Gainesville accept out-of-state medical marijuana cards? No — Florida’s MMTC program does not provide reciprocity for out-of-state medical cards.

Trulieve Phone: (352) 519-0732 Address: 1527 NW 6th St, Gainesville, FL 32601 Hours: Mon–Sat 8:30 AM–8:45 PM | Sun 9:00 AM–8:00 PM Payment: Cash, Canpay, ATM on-site Nearby neighborhoods served: Gainesville FL, Alachua County FL, Downtown Gainesville, University of Florida area, North Gainesville, Pine Park FL, Northwood FL, Newberry FL Last verified: May 2026 Notes: Florida MMTC; 1527 NW 6th St downtown Gainesville; near University of Florida campus and UF Health Shands; Trulieve in-house brands + Florida-exclusive partner brands; flower + vapes + edibles + tinctures + topicals + concentrates + RSO; Cash + Canpay + ATM; online ordering + pickup + delivery; $50 delivery minimum; $15 fee below $100; free delivery for patients 65+ with minimum order; early 8:30 AM open Mon–Sat; open 7 days. Website: trulieve.com